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0.67 mm mβcd  (Cayman Chemical)


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    Cayman Chemical 0.67 mm mβcd
    A HMGCR activity in mock and p140Cap MDA-MB-231 and SKBR3 cells; 60 nCi [14 C] HMG-CoA was added to microsomal extracts. Mevalonolactone was recovered and quantified by liquid scintillation. B HMGCR activity in tumors derived from mock and p140Cap TUBO cells orthotopically injected in Balb/c mice. C Immunoblot of immunoprecipitated total HMGCR and pSer HMGCR from mock and p140Cap MDA-MB-231 and SKBR3 cells. The ER-resident protein Calreticulin (CRT) was used as a loading control of microsomal extracts. D Quantification of mRNA levels of genes involved in the mevalonate pathway in SKBR3 p140Cap cells relative to mock cells. E Analysis of SREBP2 activity by Dual Luciferase assay in HEK293T cells transiently transfected with the indicated vectors. F Protein levels of nuclear mature SREBP2 (mSREBP2) normalized on Lamin A/C at different time points after transfection of p140Cap in HEK293T cells (see the relative western blot in Supplementary Fig. ). G , H Immunoblot showing protein levels and ubiquitination of immunoprecipitated HMGCR in control cells (CTRL), <t>following</t> <t>cholesterol</t> loading (+chol) or depletion <t>(+MβCD)</t> experiments in MDA-MB-231 and SKBR3 cells. The ER-resident CRT protein was used as a loading control. I HMGCR ubiquitination levels in MDA-MB-231 through an E3 ligases activity assay. J , K Quantifications of loading depletion experiments. Cholesterol loading assays: cells were incubated with β-methyl cyclodextrin (MβCD) then fresh medium containing cholesterol/MβCD complexes was added. Cholesterol depletion assays: cells were incubated with MβCD. Cholesterol was measured using the Cholesterol Fluorimetric Assay kit and is expressed as µmol cholesterol/mg cell proteins. L Measurement of cholesterol levels in tumors in Balb/c mice derived from mock and p140Cap TUBO cells. M Immunoblot analysis of p140Cap in microsomal fractions and whole cell lysates of MDA-MB-231, TUBO and HEK293T cell lines. The ER-resident CRT protein was used as a loading control. From ( A – L ) Unpaired test (* P < 0.05; ** P < 0.01; *** P < 0.001). Error bar: SEM.
    0.67 Mm Mβcd, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0%2E67+mm+m%CE%B2cd/pmc10733353-139-17-64?v=Cayman+Chemical
    Average 90 stars, based on 1 article reviews
    0.67 mm mβcd - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "p140Cap modulates the mevalonate pathway decreasing cell migration and enhancing drug sensitivity in breast cancer cells"

    Article Title: p140Cap modulates the mevalonate pathway decreasing cell migration and enhancing drug sensitivity in breast cancer cells

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-023-06357-z

    A HMGCR activity in mock and p140Cap MDA-MB-231 and SKBR3 cells; 60 nCi [14 C] HMG-CoA was added to microsomal extracts. Mevalonolactone was recovered and quantified by liquid scintillation. B HMGCR activity in tumors derived from mock and p140Cap TUBO cells orthotopically injected in Balb/c mice. C Immunoblot of immunoprecipitated total HMGCR and pSer HMGCR from mock and p140Cap MDA-MB-231 and SKBR3 cells. The ER-resident protein Calreticulin (CRT) was used as a loading control of microsomal extracts. D Quantification of mRNA levels of genes involved in the mevalonate pathway in SKBR3 p140Cap cells relative to mock cells. E Analysis of SREBP2 activity by Dual Luciferase assay in HEK293T cells transiently transfected with the indicated vectors. F Protein levels of nuclear mature SREBP2 (mSREBP2) normalized on Lamin A/C at different time points after transfection of p140Cap in HEK293T cells (see the relative western blot in Supplementary Fig. ). G , H Immunoblot showing protein levels and ubiquitination of immunoprecipitated HMGCR in control cells (CTRL), following cholesterol loading (+chol) or depletion (+MβCD) experiments in MDA-MB-231 and SKBR3 cells. The ER-resident CRT protein was used as a loading control. I HMGCR ubiquitination levels in MDA-MB-231 through an E3 ligases activity assay. J , K Quantifications of loading depletion experiments. Cholesterol loading assays: cells were incubated with β-methyl cyclodextrin (MβCD) then fresh medium containing cholesterol/MβCD complexes was added. Cholesterol depletion assays: cells were incubated with MβCD. Cholesterol was measured using the Cholesterol Fluorimetric Assay kit and is expressed as µmol cholesterol/mg cell proteins. L Measurement of cholesterol levels in tumors in Balb/c mice derived from mock and p140Cap TUBO cells. M Immunoblot analysis of p140Cap in microsomal fractions and whole cell lysates of MDA-MB-231, TUBO and HEK293T cell lines. The ER-resident CRT protein was used as a loading control. From ( A – L ) Unpaired test (* P < 0.05; ** P < 0.01; *** P < 0.001). Error bar: SEM.
    Figure Legend Snippet: A HMGCR activity in mock and p140Cap MDA-MB-231 and SKBR3 cells; 60 nCi [14 C] HMG-CoA was added to microsomal extracts. Mevalonolactone was recovered and quantified by liquid scintillation. B HMGCR activity in tumors derived from mock and p140Cap TUBO cells orthotopically injected in Balb/c mice. C Immunoblot of immunoprecipitated total HMGCR and pSer HMGCR from mock and p140Cap MDA-MB-231 and SKBR3 cells. The ER-resident protein Calreticulin (CRT) was used as a loading control of microsomal extracts. D Quantification of mRNA levels of genes involved in the mevalonate pathway in SKBR3 p140Cap cells relative to mock cells. E Analysis of SREBP2 activity by Dual Luciferase assay in HEK293T cells transiently transfected with the indicated vectors. F Protein levels of nuclear mature SREBP2 (mSREBP2) normalized on Lamin A/C at different time points after transfection of p140Cap in HEK293T cells (see the relative western blot in Supplementary Fig. ). G , H Immunoblot showing protein levels and ubiquitination of immunoprecipitated HMGCR in control cells (CTRL), following cholesterol loading (+chol) or depletion (+MβCD) experiments in MDA-MB-231 and SKBR3 cells. The ER-resident CRT protein was used as a loading control. I HMGCR ubiquitination levels in MDA-MB-231 through an E3 ligases activity assay. J , K Quantifications of loading depletion experiments. Cholesterol loading assays: cells were incubated with β-methyl cyclodextrin (MβCD) then fresh medium containing cholesterol/MβCD complexes was added. Cholesterol depletion assays: cells were incubated with MβCD. Cholesterol was measured using the Cholesterol Fluorimetric Assay kit and is expressed as µmol cholesterol/mg cell proteins. L Measurement of cholesterol levels in tumors in Balb/c mice derived from mock and p140Cap TUBO cells. M Immunoblot analysis of p140Cap in microsomal fractions and whole cell lysates of MDA-MB-231, TUBO and HEK293T cell lines. The ER-resident CRT protein was used as a loading control. From ( A – L ) Unpaired test (* P < 0.05; ** P < 0.01; *** P < 0.001). Error bar: SEM.

    Techniques Used: Activity Assay, Derivative Assay, Injection, Western Blot, Immunoprecipitation, Luciferase, Transfection, Incubation, Fluorimetry Assay



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    0.67 mm mβcd  (Cayman Chemical)
    90
    Cayman Chemical 0.67 mm mβcd
    A HMGCR activity in mock and p140Cap MDA-MB-231 and SKBR3 cells; 60 nCi [14 C] HMG-CoA was added to microsomal extracts. Mevalonolactone was recovered and quantified by liquid scintillation. B HMGCR activity in tumors derived from mock and p140Cap TUBO cells orthotopically injected in Balb/c mice. C Immunoblot of immunoprecipitated total HMGCR and pSer HMGCR from mock and p140Cap MDA-MB-231 and SKBR3 cells. The ER-resident protein Calreticulin (CRT) was used as a loading control of microsomal extracts. D Quantification of mRNA levels of genes involved in the mevalonate pathway in SKBR3 p140Cap cells relative to mock cells. E Analysis of SREBP2 activity by Dual Luciferase assay in HEK293T cells transiently transfected with the indicated vectors. F Protein levels of nuclear mature SREBP2 (mSREBP2) normalized on Lamin A/C at different time points after transfection of p140Cap in HEK293T cells (see the relative western blot in Supplementary Fig. ). G , H Immunoblot showing protein levels and ubiquitination of immunoprecipitated HMGCR in control cells (CTRL), <t>following</t> <t>cholesterol</t> loading (+chol) or depletion <t>(+MβCD)</t> experiments in MDA-MB-231 and SKBR3 cells. The ER-resident CRT protein was used as a loading control. I HMGCR ubiquitination levels in MDA-MB-231 through an E3 ligases activity assay. J , K Quantifications of loading depletion experiments. Cholesterol loading assays: cells were incubated with β-methyl cyclodextrin (MβCD) then fresh medium containing cholesterol/MβCD complexes was added. Cholesterol depletion assays: cells were incubated with MβCD. Cholesterol was measured using the Cholesterol Fluorimetric Assay kit and is expressed as µmol cholesterol/mg cell proteins. L Measurement of cholesterol levels in tumors in Balb/c mice derived from mock and p140Cap TUBO cells. M Immunoblot analysis of p140Cap in microsomal fractions and whole cell lysates of MDA-MB-231, TUBO and HEK293T cell lines. The ER-resident CRT protein was used as a loading control. From ( A – L ) Unpaired test (* P < 0.05; ** P < 0.01; *** P < 0.001). Error bar: SEM.
    0.67 Mm Mβcd, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0%2E67+mm+m%CE%B2cd/pmc10733353-139-17-64?v=Cayman+Chemical
    Average 90 stars, based on 1 article reviews
    0.67 mm mβcd - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A HMGCR activity in mock and p140Cap MDA-MB-231 and SKBR3 cells; 60 nCi [14 C] HMG-CoA was added to microsomal extracts. Mevalonolactone was recovered and quantified by liquid scintillation. B HMGCR activity in tumors derived from mock and p140Cap TUBO cells orthotopically injected in Balb/c mice. C Immunoblot of immunoprecipitated total HMGCR and pSer HMGCR from mock and p140Cap MDA-MB-231 and SKBR3 cells. The ER-resident protein Calreticulin (CRT) was used as a loading control of microsomal extracts. D Quantification of mRNA levels of genes involved in the mevalonate pathway in SKBR3 p140Cap cells relative to mock cells. E Analysis of SREBP2 activity by Dual Luciferase assay in HEK293T cells transiently transfected with the indicated vectors. F Protein levels of nuclear mature SREBP2 (mSREBP2) normalized on Lamin A/C at different time points after transfection of p140Cap in HEK293T cells (see the relative western blot in Supplementary Fig. ). G , H Immunoblot showing protein levels and ubiquitination of immunoprecipitated HMGCR in control cells (CTRL), following cholesterol loading (+chol) or depletion (+MβCD) experiments in MDA-MB-231 and SKBR3 cells. The ER-resident CRT protein was used as a loading control. I HMGCR ubiquitination levels in MDA-MB-231 through an E3 ligases activity assay. J , K Quantifications of loading depletion experiments. Cholesterol loading assays: cells were incubated with β-methyl cyclodextrin (MβCD) then fresh medium containing cholesterol/MβCD complexes was added. Cholesterol depletion assays: cells were incubated with MβCD. Cholesterol was measured using the Cholesterol Fluorimetric Assay kit and is expressed as µmol cholesterol/mg cell proteins. L Measurement of cholesterol levels in tumors in Balb/c mice derived from mock and p140Cap TUBO cells. M Immunoblot analysis of p140Cap in microsomal fractions and whole cell lysates of MDA-MB-231, TUBO and HEK293T cell lines. The ER-resident CRT protein was used as a loading control. From ( A – L ) Unpaired test (* P < 0.05; ** P < 0.01; *** P < 0.001). Error bar: SEM.

    Journal: Cell Death & Disease

    Article Title: p140Cap modulates the mevalonate pathway decreasing cell migration and enhancing drug sensitivity in breast cancer cells

    doi: 10.1038/s41419-023-06357-z

    Figure Lengend Snippet: A HMGCR activity in mock and p140Cap MDA-MB-231 and SKBR3 cells; 60 nCi [14 C] HMG-CoA was added to microsomal extracts. Mevalonolactone was recovered and quantified by liquid scintillation. B HMGCR activity in tumors derived from mock and p140Cap TUBO cells orthotopically injected in Balb/c mice. C Immunoblot of immunoprecipitated total HMGCR and pSer HMGCR from mock and p140Cap MDA-MB-231 and SKBR3 cells. The ER-resident protein Calreticulin (CRT) was used as a loading control of microsomal extracts. D Quantification of mRNA levels of genes involved in the mevalonate pathway in SKBR3 p140Cap cells relative to mock cells. E Analysis of SREBP2 activity by Dual Luciferase assay in HEK293T cells transiently transfected with the indicated vectors. F Protein levels of nuclear mature SREBP2 (mSREBP2) normalized on Lamin A/C at different time points after transfection of p140Cap in HEK293T cells (see the relative western blot in Supplementary Fig. ). G , H Immunoblot showing protein levels and ubiquitination of immunoprecipitated HMGCR in control cells (CTRL), following cholesterol loading (+chol) or depletion (+MβCD) experiments in MDA-MB-231 and SKBR3 cells. The ER-resident CRT protein was used as a loading control. I HMGCR ubiquitination levels in MDA-MB-231 through an E3 ligases activity assay. J , K Quantifications of loading depletion experiments. Cholesterol loading assays: cells were incubated with β-methyl cyclodextrin (MβCD) then fresh medium containing cholesterol/MβCD complexes was added. Cholesterol depletion assays: cells were incubated with MβCD. Cholesterol was measured using the Cholesterol Fluorimetric Assay kit and is expressed as µmol cholesterol/mg cell proteins. L Measurement of cholesterol levels in tumors in Balb/c mice derived from mock and p140Cap TUBO cells. M Immunoblot analysis of p140Cap in microsomal fractions and whole cell lysates of MDA-MB-231, TUBO and HEK293T cell lines. The ER-resident CRT protein was used as a loading control. From ( A – L ) Unpaired test (* P < 0.05; ** P < 0.01; *** P < 0.001). Error bar: SEM.

    Article Snippet: In cholesterol loading assays, 5 × 10 6 cells (after overnight starvation) were incubated with 0.67 mM MβCD for 4 h, then washed; fresh medium containing 0.5 mM cholesterol/MβCD complexes was added for 24 h. In cholesterol depletion assays, cells were incubated with 0.67 mM MβCD for 4 h. After these incubation times, the cellular cholesterol was measured using the Cholesterol Fluorimetric Assay kit (Cayman Chemical, Ann Arbor, MI) as per the manufacturer’s instructions.

    Techniques: Activity Assay, Derivative Assay, Injection, Western Blot, Immunoprecipitation, Luciferase, Transfection, Incubation, Fluorimetry Assay